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cd138 microbeads, human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd138 microbeads, human
    Cd138 Microbeads, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd138+microbeads/CD138+MicroBeads%2C+human/custom%40130-051-301%4042767467
    Average 96 stars, based on 208 article reviews
    cd138 microbeads, human - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism
    Article Snippet: .. To separate the CD138+ cells (= MM cells) from the CD138‐ cells, cells were labelled with human CD138 Microbeads (order no. 130–051‐301, Miltenyi Biotec, Gladbach, Germany) and isolated by magnetically activated cell sorting according to the manufacturer's instructions. ..

    Article Title: System vaccinology analysis of predictors and mechanisms of antibody response durability to multiple vaccines in humans.
    Article Snippet: Mario Cortese, Thomas Hagan , Nadine Rouphael , Sheng-Yang Wu, Xia Xie, Dmitri Kazmin, Florian Wimmers , Shakti Gupta, Robbert van der Most , Margherita Coccia , Prabhu S. Aranuchalam , Helder I. Nakaya , Yating Wang, Elizabeth Coyle, Shu Horiuchi, Hanchih Wu, Mary Bower, Aneesh Mehta, Clifford Gunthel, Steve E. Bosinger , Yuri Kotliarov , Foo Cheung, Pamela L. Schwartzberg , Ronald N. Germain , John Tsang , Shuzhao Li, Randy Albrecht , Hideki Ueno , Shankar Subramaniam, Mark J. Mulligan, Surender Khurana , Hana Golding & Bali Pulendran

    Article Title: PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism.
    Article Snippet: .. To separate the CD138+ cells (= MM cells) from the CD138- cells, cells were labelled with human CD138 Microbeads (order no. 130–051-301, Miltenyi Biotec, Gladbach, Germany) and isolated by magnetically activated cell sorting according to the manufacturer’s instructions. ..

    Article Title: Syntenin inhibition impairs stroma‐tumor communication in multiple myeloma and improves bortezomib treatment efficiency
    Article Snippet: .. CD138 + BM cell fractions were isolated using magnetic activated cell sorting using human CD138 MicroBeads (Miltenyi Biotec, Gladbach, Germany). .. CD138 − and CD138 + cells were mixed (in 1:1 or 2:1) and treated with SyntOFF (50 or 100 μm) alone or combined with bortezomib for 24 h. BM cells were stained with CD138‐APC (130‐091‐250) (Miltenyi Biotec, Gladbach, Germany) and detected by flow cytometry.

    Article Title: Unknown Immunoregulatory Effects of FcRn Inhibition by Efgartigimod in Myasthenia Gravis
    Article Snippet: PBMCs were analyzed by flow cytometry immediately after isolation; the remaining cells were frozen in FBS plus 10% dimethyl sulfoxide (Euroclone) and stored in liquid nitrogen for subsequent molecular analyses. .. CD138+ plasma cells were positively selected and isolated from PBMCs using human CD138 MicroBeads (Miltenyi Biotec). .. In brief, at least 5 × 10 6 PBMCs were incubated with the CD138 MicroBeads, according to the manufacturer's instructions, and then passed through a Magnetic Activated Cell Sorting (MACS) column in the magnetic field of a MACS separator.

    Article Title: Selective Enhancer Gain of Function Deregulates MYC Expression in Multiple Myeloma
    Article Snippet: .. CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). ..

    Article Title: System vaccinology analysis of predictors and mechanisms of antibody durability to multiple vaccines in humans
    Article Snippet: .. Plasma cells were isolated from BMNC using human CD138 microbeads (Miltenyi, 130–051-301) following the manufacturer’s protocol. .. CD138 - cells were further incubated with human CD61 microbeads (Miltenyi, 130–051-101) for megakaryocyte isolation.

    FACS:

    Article Title: PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism
    Article Snippet: .. To separate the CD138+ cells (= MM cells) from the CD138‐ cells, cells were labelled with human CD138 Microbeads (order no. 130–051‐301, Miltenyi Biotec, Gladbach, Germany) and isolated by magnetically activated cell sorting according to the manufacturer's instructions. ..

    Article Title: PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism.
    Article Snippet: .. To separate the CD138+ cells (= MM cells) from the CD138- cells, cells were labelled with human CD138 Microbeads (order no. 130–051-301, Miltenyi Biotec, Gladbach, Germany) and isolated by magnetically activated cell sorting according to the manufacturer’s instructions. ..

    Article Title: Syntenin inhibition impairs stroma‐tumor communication in multiple myeloma and improves bortezomib treatment efficiency
    Article Snippet: .. CD138 + BM cell fractions were isolated using magnetic activated cell sorting using human CD138 MicroBeads (Miltenyi Biotec, Gladbach, Germany). .. CD138 − and CD138 + cells were mixed (in 1:1 or 2:1) and treated with SyntOFF (50 or 100 μm) alone or combined with bortezomib for 24 h. BM cells were stained with CD138‐APC (130‐091‐250) (Miltenyi Biotec, Gladbach, Germany) and detected by flow cytometry.

    Clinical Proteomics:

    Article Title: System vaccinology analysis of predictors and mechanisms of antibody response durability to multiple vaccines in humans.
    Article Snippet: Mario Cortese, Thomas Hagan , Nadine Rouphael , Sheng-Yang Wu, Xia Xie, Dmitri Kazmin, Florian Wimmers , Shakti Gupta, Robbert van der Most , Margherita Coccia , Prabhu S. Aranuchalam , Helder I. Nakaya , Yating Wang, Elizabeth Coyle, Shu Horiuchi, Hanchih Wu, Mary Bower, Aneesh Mehta, Clifford Gunthel, Steve E. Bosinger , Yuri Kotliarov , Foo Cheung, Pamela L. Schwartzberg , Ronald N. Germain , John Tsang , Shuzhao Li, Randy Albrecht , Hideki Ueno , Shankar Subramaniam, Mark J. Mulligan, Surender Khurana , Hana Golding & Bali Pulendran

    Article Title: Unknown Immunoregulatory Effects of FcRn Inhibition by Efgartigimod in Myasthenia Gravis
    Article Snippet: PBMCs were analyzed by flow cytometry immediately after isolation; the remaining cells were frozen in FBS plus 10% dimethyl sulfoxide (Euroclone) and stored in liquid nitrogen for subsequent molecular analyses. .. CD138+ plasma cells were positively selected and isolated from PBMCs using human CD138 MicroBeads (Miltenyi Biotec). .. In brief, at least 5 × 10 6 PBMCs were incubated with the CD138 MicroBeads, according to the manufacturer's instructions, and then passed through a Magnetic Activated Cell Sorting (MACS) column in the magnetic field of a MACS separator.

    Article Title: System vaccinology analysis of predictors and mechanisms of antibody durability to multiple vaccines in humans
    Article Snippet: .. Plasma cells were isolated from BMNC using human CD138 microbeads (Miltenyi, 130–051-301) following the manufacturer’s protocol. .. CD138 - cells were further incubated with human CD61 microbeads (Miltenyi, 130–051-101) for megakaryocyte isolation.

    other:

    Article Title: Unknown Immunoregulatory Effects of FcRn Inhibition by Efgartigimod in Myasthenia Gravis
    Article Snippet: Anti-AChR Abs were assessed in serum by radioimmunoprecipitation.25 PBMC Isolation PBMCs were isolated from fresh whole blood using the FicollPaque PLUS (Cytiva) gradient centrifugation protocol; cell number was estimated by the Trypan blue (Sigma-Aldrich) dye exclusion method.



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    (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), <t>CD138</t> (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).
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    (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), <t>CD138</t> (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).
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    Image Search Results


    Co-injection of human mast cells with primary CD138 MM cells leads to faster engraftment and bone destruction in the NSG-hIL6 MM PDX model.

    Journal: bioRxiv

    Article Title: Mast Cells Enhance Myeloma Engraftment and Promote Bone Destruction in the NSG-hIL6 Patient Derived Xenograft Model

    doi: 10.64898/2026.05.14.725220

    Figure Lengend Snippet: Co-injection of human mast cells with primary CD138 MM cells leads to faster engraftment and bone destruction in the NSG-hIL6 MM PDX model.

    Article Snippet: Fresh cells were subjected to CD138+ bead selection (Miltenyi 130-111-744) on LS columns (Miltenyi 30-042-401) according to manufacturer instructions and checked for purity by flow cytometry using singlet, live/dead (Thermo L10119) and intracellular light chain staining (Supplemental Figure 2).

    Techniques: Injection

    (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), CD138 (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).

    Journal: medRxiv

    Article Title: Single-Cell Analysis Reveals Inflammatory–Immunosuppressive Niches in Daratumumab-Resistant Primary AL Amyloidosis

    doi: 10.64898/2026.03.28.26349317

    Figure Lengend Snippet: (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), CD138 (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).

    Article Snippet: PC enrichment was performed using CD138 + Magnetic Beads (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat# 130-051-301), MS columns (Cat# 130-042-201), and a MiniMACS separator (Cat# 130-042-102).

    Techniques: Multiplex Assay, Immunofluorescence, Biomarker Discovery, Activation Assay